![]() ![]() Dicer-dependent and -independent Argonaute2 protein interaction networks in mammalian cells. Hsc70/Hsp90 chaperone machinery mediates ATP-dependent RISC loading of small RNA duplexes. In vitro assembly of plant RNA-induced silencing complexes facilitated by molecular chaperone HSP90. HSP90 protein stabilizes unloaded argonaute complexes and microscopic P-bodies in human cells. siRNA repositioning for guide strand selection by human Dicer complexes. The role of PACT in the RNA silencing pathway. TRBP, a regulator of cellular PKR and HIV-1 virus expression, interacts with Dicer and functions in RNA silencing. TRBP recruits the Dicer complex to AGO2 for microRNA processing and gene silencing. Tomari, Y., Matranga, C., Haley, B., Martinez, N. R2D2, a bridge between the initiation and effector steps of the Drosophila RNAi pathway. Normal microRNA maturation and germ-line stem cell maintenance requires loquacious, a double-stranded RNA-binding domain protein. Human RISC couples microRNA biogenesis and posttranscriptional gene silencing. A human, ATP-independent, RISC assembly machine fueled by pre-miRNA. Identification of novel Argonaute-associated proteins. Asymmetry in the assembly of the RNAi enzyme complex. Functional siRNAs and miRNAs exhibit strand bias. Reference 11 identifies a fourth catalytic residue defining a catalytic tetrad. Reference 16 solves the structure in the presence of tryptophan and provides a model for TRP binding on the surface of the PIWI domain, which is used by G182 proteins to interact with AGO proteins. Reference 15 solves the structure of human AGO2 together with a bound miRNA. References 11, 15 and 16 solve the crystal structures of full-length eukaryotic AGO proteins. The crystal structure of human Argonaute2. The structure of human Argonaute-2 in complex with miR-20a. Turning catalytically inactive human Argonaute proteins into active slicer enzymes. References 12 and 13 show that AGO2 is the catalytic subunit of human RISC that cleaves target RNAs complementary to the bound siRNA or miRNA. Human Argonaute2 mediates RNA cleavage targeted by miRNAs and siRNAs. Argonaute2 is the catalytic engine of mammalian RNAi. Structure of yeast Argonaute with guide RNA. The N domain of Argonaute drives duplex unwinding during RISC assembly. Recognition of 2′- O-methylated 3′-end of piRNA by the PAZ domain of a Piwi protein. A three-dimensional view of the molecular machinery of RNA interference. Multiple roles for small RNAs during plant reproduction. elegans Argonaute family reveals that distinct Argonautes act sequentially during RNAi. PIWI-interacting small RNAs: the vanguard of genome defence. Argonaute proteins: key players in RNA silencing. Argonaute proteins: mediators of RNA silencing. ![]()
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